We are experts in looking for and dosing active ingredients by acknowledging q matrixes.
We check for limited and allowed contents and we endorse protocols using the standard addition method.

  • Coumarin and furocoumarins

Quantification of coumarins in natural extract (Tonka broad beans, Melilot, Lavander essential oil, aromatic preparation of aniseed beverage, Pastis…)
Some of furocoumarins and psoralens are photosensitive. determination of the content of these molecules  is thus necessary in the preparations likely to contain some.
Quantification of bergapten in essentials oils of bergamot (fucoumarin free or not)
Screening for furocoumarins in essential oil and in perfumed solutions. Main coumarins and fucoumarins are Bergamottin, Epoxybergamottin, Bergapten, Byakangelicol, 7-Methoxycoumarin (herniarin), 5,7-Dimethoxycoumarin (citropten), Imperatorin, Isopimpinellin, Oxypeucedanin, Psoralen, Trioxalen, Xanthotoxin, 6-Methylcoumarin (Toncarine), SAME (5-geranoxy-7-methoxycoumarine), trioxalen, angelicin, 5-hydroxypsoralen (bergaptol), 8- hydroxypsoralen (xanthotoxol), 5-methoxyangelicin (isobergapten)…
Extraction and HPLC-DAD analysis of the 15 furocoumarins from IFRA list: Psoralen, Bergapten, Xanthotoxin, Isopimpinellin, Oxypeucedanin, Oxypeucedanin hydrate, Byakangelicol, Byakangelicin, Heraclenin, 8-geranyloxypsoralen, Bergamottin, Imperatorin, Isoimperatorin, phellopterin and Epoxybergamottin

GC Profile (polar and apolar columns)
Relative percentages of main components, characteristics of essential oils Conformity with ISO norms
Essential oil content
by steam distillation of aromatic plants
Main essential oils analysed
chinus molle, basil, bergamot, cananga, cinnamon (bark and leaf), citronnella, ginger, clove (bud, leaf, stem), lavender, palmarosa, patchouli, citrus (peal and leaf), pimento (berry and leaf), pepper, raventsara, tuberose, ylang ylang…
Realization of concretes and absolutes (to labotary scales) and characterisation
Iris: Roots, resinoïds, butter: Quantification of free irones, determination of irones potential
Clary sage: concretes, absolutes, herbs
Quantification of sclareol, epi-sclaréol and sclareolid
Tree moss – Oak moss: concretes, absolutes
Quantification of atranorin, chloratranorin, atranol, ethyl chhloratranol, ethyl hemmatomate evernyl, chlorevernyl, isopropyle hemmatomate.

Germs content
Aerobic mesophile flora,thermo tolerent coliforms, escherichia coli, Staphylococca with positive coagulase. Anaerobe sulfito reduceurs, Yeats, moulds, salmonella…
Detection and content of aflatoxins
By CHLP, aflatoxins B1, B2, G1 and G2

Plum, Cherry plum, Quetsche, Cherry, Apricot, Prune, Peach
Determination of cyanhydric acid content using colouring method :

  • in fruit spirit, spirit, infusion, maceration, extract, preserve
  • white brandy may be analysed directly, in the case of coloured brandy, it is necessary to distillate first.

Regulation EEC n° 1576/89 :

  • Cyanhydric acid content is limited in alcoholic beverages and beverages…
  • cyanhydric acid content is limited in kernel fruits preserves.

Strawberry, Raspberry, Blackcurrant, Grape, Blueberry, Currant, Blackberry, Cherry, Orange
Anthocyanins are an important family of flavonoïds. They are responsible for red and purplish colourings in fruits and flowers. The analysis of anthocyanins consists in determining which red fruits are present in a preparation (juice, syrup, jam…)It is a qualitative analysis, carried out by HPLC. It enables the detection of additions of elderberry to black currant juice or a syrup of grenadine for example.

Determination of glycyrrhizic acid content
Method by HPLC in liquorice (Glycyrrhyza glabra L.): root, infusion, extract, powder, block, confectionery, pharmaceutical use, alcoolic beverage, Pastis.
The results are expressed with mono ammonium glycyrrhizic acid pure standard, used by DGCCRF laboratory of Montpellier for the control of flavoured spirit drinks. Labelling of main foodstuffs containing glycyrrhizic acid: particular labelling due to possible rise of hypertension (Directive CE n° 2004/77)
Glycyrrhetic acid content (isomers 18α and 18β), Enoxolone
Uses: pharmaceutical, veterinary, cosmetic

Spirit drinks- Spirituous beverage – Pastis
Reference methods for the analysis of spirits drinks (CE n°2091/2002):

  • Determination of trans anethole in aniseed-flavoured spirit drinks
  • Determination of glycyrrhizic acid and chalcones in Pastis
  • Determination of thujones in spirit drinksAlternative  HPLC method for determination of trans-anethole content
    International methods for the analysis of spirit drinks, alcohols and aromatic fraction of beverages (OIV collection)
    alcoholic strength, sugar content, dry matter, acid values, hydroxy methyl furfural, chromatic values, colouring values, cyanhydric ion, synthetic dyes, limited compounds, isotopic ratio..

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